Electrophysiology

 


WinWCP & WinEDR data acquisition and analysis program for handling signals from electrophysiological experiments


WinWCP & WinEDR are part of the public domain Strathclyde Electrophysiology Software package, supplied free of charge to academic users by Dr. John Dempster, of the University of Strathclyde in Glasgow, U.K.

WinWCP

WinWCP is a data acquisition and analysis program for handling signals from whole-cell electrophysiological experiments. These may include whole-cell patch clamp experiments, single- and two-microelectrode voltage-clamp studies, or simple membrane potential recordings. Whole-cell signals are produced by the summation of currents through the (usually) large population of ion channels in the cell membrane, and thus consist of relatively smooth current or potential waveforms. The amplitude and time course of such signals contain information concerning the kinetic behaviour of the underlying ion channels, and other cellular processes, which can be extracted by the application of a variety of waveform analysis techniques.

winwcp_1.gif (48030 bytes)
Please click to enlarge

winwcp_2.gif (39471 bytes)
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WinWCP provides, in a single program, the data acquisition and experimental stimulus generation features necessary to make a digital recording of the electrophysiological signals, and a range of waveform analysis procedures commonly applied to such signals. WinWCP acts like a multi-channel digital oscilloscope, collecting series of signal and storing them in a data file on magnetic disk. Its major features are:

Recording

  • 8 analogue input channels.
  • 29952 samples per recording sweep.
  • 2 billion records per data file.
  • Stimulus voltage waveform generator.
  • 8 TTL digital output lines, for operating solenoid controlled valves or other experimental devices.
  • TTL External trigger input, to synchronise recording sweeps with external events.
  • Digital valve control pattern generator.
  • Spontaneous event detector.

Analysis

  • Signal averaging.
  • Leak current subtraction.
  • Automatic waveform amplitude/time course measurement.
  • Mathematical curve fitting to waveforms.
  • Non-stationary noise analysis.
  • Quantal analysis of synaptic currents.
  • Synaptic driving function analysis.Synaptic current and Hodgkin-Huxley current simulations.

Download (please see the conditions of use)

See here

WinEDR

WinEDR is a data acquisition and analysis program for handling signals from electrophysiological experiments. These may include whole-cell patch clamp experiments, single- and two-microelectrode voltage-clamp studies, or simple membrane potential recordings. Whole -cell signals are produced by the summation of currents through the (usually) large population of ion channels in the cell membrane, and thus consist of relatively smooth current or potential waveforms. The amplitude and time course of such signals contain information concerning the kinetic behaviour of the underlying ion channels, and other cellular processes, which can be extracted by the application of a variety of waveform analysis techniques.

winedr_1.gif (44911 bytes)
Please click to enlarge

WinEDR provides, in a single program, the data acquisition and experimental stimulus generation features necessary to make a digital recording of the electrophysiological signals, and a range of waveform analysis procedures commonly applied to such signals. WinEDR acts like a multi-channel digital tape recorder, collecting series of signals and storing them in a data file on magnetic disk. Its major features are

Recording

  • 8 analogue input channels.
  • Continuous sampling-to-disc at rates up to 50 kHz.
  • Stimulus generator.

Analysis

  • Single-channel transition detection and amplitude/dwell time analysis.
  • Event detection and frequency analysis
  • Noise analysis
  • Miniature synaptic current simulations.

Download (please see the conditions of use)

See here

Hardware

For new installations using analog Patch-Clamp amplifier (e.g. RK-400), we recommend boards from National Instruments. They are easy to install and come with good support for the various versions of Windows. In particular, consider:

  • PCI-6014 low cost board. It has a 16 bit ADC and can sample at 200 kHz. Waveform generation update rates are limited to 1kHz due to the lack of DMA channel for D/A data transfer.
    PCI-6014 board
  • PCI-MIO-16E-4 (or PCI 6040E). It has a 12 bit ADC and samples at rates up to 500 kHz. Both A/D and D/A data transfer are carried out using DMA channels.
  • PCI-6052 fast high end 16 bit board.

National Instruments supply a range of I/O panels with BNC sockets. The BNC 2090 is designed to be mounted in a 19" rack whereas the BNC 2110 is smaller free standing panel. Both are connected to the cards using shielded 68 way (68-68SH) cables.
BNC 2110

Please contact us if you need a quotation.

Conditions of use.

WinWCP & WinEDR Software package is a suite of programs for the acquisition and analysis of electrophysiological signals, developed by the author at the department of Physiology & Pharmacology, University of Strathclyde.

At the discretion of the author, the software is supplied free of charge to academic users and others working for non-commercial, non-profit making, organisations. Commercial organisations may purchase a license to use the software from the University of Strathclyde (contact the author for details).

The author retains copyright and all rights are reserved. The user may use the software freely for their own research, but should not sell or pass the software on to others without the permission of the author.

Except where otherwise specified, no warranty is implied, by either the author or the University of Strathclyde, concerning the fitness of the software for any purpose.

The software is supplied "as found" and the user is advised to verify that the software functions appropriately for the purposes that they choose to use it.

An acknowledgement of the use of the software, in publications to which it has contributed, would be gratefully apprecia ted by the author.

John Dempster
Department of Physiology & Pharmacology
Strathclyde Institute for Biomedical Sciences
University of Strathclyde
27 Taylor St.
GLASGOW G4 0NR
Scotland
Tel (0)141 548 2320
Fax (0)141 552 2562
E-mail j.dempster@strath.ac.uk
Web

Note: the information contained on this page is provided courtesy of the author John Dempster with his written permission.

 

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