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General features of the RK-400
- The intrinsic noise is close to the minimal theoretical noise, and a large bandwidth (50
kHz) is available with a uniform frequency response on resistor
headstage. A gain from 1 to 1000 by steps of 1, 2, 5 can be applied to the headstage
signal.
- It can run in voltage clamp (VClamp) or current clamp (IClamp) mode, with distinct
holding and input commands, and it can be switched from VClamp to IClamp, either manually
or through a remote command signal. Transient compensations are switched automatically off
when going to current clamp mode, to prevent oscillations.
- Two digital displays simultaneously indicate the voltage and current.
- Transient and leak neutralizations offer advantage of optimal headstage amplification.
- A series resistance compensation circuit and a supercharging circuit permits a
satisfactory control of the potential or current to the preparation.
- Additional features such as a duration adjustable signal (zap) to penetrate cells, a 5
poles overshoot free (Bessel) anti-aliasing adjustable filter, are provided.
- Gain telegraph output (compatible P-Clamp)
- Delivered with a dual resistor headstage HK-R-410/408 (10 GOhm/100 MOhm)
and a PC S3 electrode holder.
The RK-400 appears as a 19" amplifier box 2U high linked to the amplifier
headstage. A complete manual allows a fast mastering of the system.
Specifications
| Head-Stage |
Model HK-R-410/08 : Dual resistor Head-stage (10 GOhm/100 MOhm)
|
| Gain Switch |
- 10 to 10000 mV/pA (10 GOhm resistor feedback)
- 0.1 to 100 mV/pA (100 MOhm resistor feedback)
|
| Frequency response |
Internally limited to 50 kHz in all modes |
| Measurement Range |
Integrator : see below
Resistance mode 10 nA x (1/R) (R = feedback resistor in GOhm) |
| Noise |
Resistor feedback :
- 10 GOhm : 60 fA rms at 1 kHz, 0.6 pA rms at 10 kHz
- 100 MOhm : 0.45 pA rms at 1 kHz, 1.8 pA rms at 10 kHz
(Measured with the 5-pole Bessel filter)
|
| Operating Modes |
Voltage-clamp, Current-clamp to zero current, Current-clamp with commands, Search
(Vtrack), external switching of V/I clamp |
| Internal Holding Commands |
Separate voltage and current setting for voltage-clamp mode and current-clamp mode.
Voltage-clamp ± 0 to 200 mV
Current-clamp (Range dependent on head-stage gain, ex : 1 nA/V for capacitor and 1 GOhm
feedback) |
| Junction Zeroing |
± 200 mV |
| Input for External Commands |
Two input ports for voltage command (scaling 20 mV/V)
Current command. (Scaling dependent on head-stage gain) |
| Internal Pulse Generator |
1 mV and 10 mV square wave, operative in V-clamp mode only, Zap signal for cell
penetration |
| Leak Neutralization |
Leak conductance (range dependent on head-stage gain.) (infinite to 50 MOhm) |
| Capacitances Transient Neutralization |
Electrode fast compensation : 0 to 10 pF, 0.5 to 5 ms
Electrode slow compensation : 0 to 1 pF, 0.1 to 10 ms
Cell parameters neutralization : 0.5 to 100 MOhm of series resistance, 1 to 100 pF of cell
capacitance |
| Series Resistance Compensation |
0 to 100 % of the series resistance
1 to 100 microsecond lag |
| Low-Pass Filter |
Active 5-pole Bessel filter : 0.3, 1, 3, and 10 kHz
Direct output with 50 kHz bandpass. |
| Digital Voltmeters |
Two 4 1/2 digit liquid crystal. One for current, and one for voltage measurements. |
| Power Supply |
220/110 V - 50/60 Hz
Doubly regulated power supply |
| Weight |
3 kg |
* ExWork price subject to change without prior
notice. Please contact us for a quotation.
References
- Selection of scientific articles using the
Bio-Logic Patch-Clamp amplifiers - Click here
Software
The RK-400 can be controlled by different software. However we recommend
WinWCP & WinEDR.
WinWCP & WinEDR
are part of the public domain Strathclyde Electrophysiology
Software package, supplied free of charge to academic users by Dr. John Dempster,
of the University of Strathclyde in Glasgow, U.K.
Please click to enlarge
Please click to enlarge |
